Molecular Properties of Lysostaphin, a Bacteriolytic Agent Specific for Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
Molecular properties of lysostaphin, a bacteriolytic agent specific for Staphylococcus aureus.
Lysostaphin is a zinc-metalloenzyme which has a molecular weight of 25,000 when determined by sedimentation equilibrium in dilute aqueous salt solutions. Combination of the sedimentation coefficient, sZO,lo = 2.32, and diffusion coefficient, DBo,w = 7.83, gave a native molecular weight of 25,800. Sedimentation equilibrium of the protein in 6 M guanidine hydrochloride, with and without 2-mercapt...
متن کاملLysostaphin: a New Bacteriolytic Agent for the Staphylococcus.
3Wilt, F. H., Biochem. Biophys. Res. Commun., 11, 447 (1963). 7Brachet, J., et al., Biochim. Biophys. Acta, 72, 662 (1963). 8 Gross, P. R., and G. H. Cousineau, Exptl. Cell Res., in press. I Hultin, T., Exptl. Cell Res., 25, 405 (1961). 10 Wilt, F. H., and T. Hultin, Biochem. Biophys. Res. Commun., 9, 313 (1962). 11 Melton, C. R., Genetics, 48, 901 (1963). 12Tyler, A., Am. Zoologist, 3, 109 (19...
متن کاملLysostaphin-induced, osmotically fragile Staphylococcus aureus cells.
Staphylococcus aureus FDA 209P cells when suspended in 24% (w/v) NaCl were rendered osmotically fragile by exposure to lysostaphin for time intervals ranging from 2 to 60 min. Such cells were analyzed chemically and serologically for evidence of residual cell wall material, were plated in hypertonic sucrose medium to determine revertibility to normal, and were subjected to manometric studies to...
متن کاملTransfection of lysostaphin-treated cells of Staphylococcus aureus.
After treatment with 1 unit of lysostaphin per ml for 3 min, two strains of Staphylococcus aureus, 233 and PS 44A HJD, were transfected with phenol-extracted deoxyribonucleic acid (DNA) from the staphylococcal bacteriophages, 53 and 44A HJD, respectively. The number of transfected cells was low in both systems, approximately two in 10(7) enzyme-treated cells. There was a saturation effect at hi...
متن کاملRapid identification of Staphylococcus aureus by using lysostaphin sensitivity.
Lysostaphin sensitivity was evaluated as a rapid screening test to differentiate Staphylococcus aureus from other species of staphylococci and micrococci. A total of 168 strains of staphylococci, 108 of which were S. aureus, were cultured overnight in brain infusion broth. Gram stains were peformed before and after a 1:10 dilution of the culture was exposed to 2 micrograms of lysostaphin per ml...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62869-8